ASPC-1-Luc
CBP30142L
| I. Introduction | |
| Host Cell: | ASPC-1 |
| Expressed gene: | Luciferase |
| Stability: | 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+2μg/ml Puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen |
| II. Description of Host Cell Line | |
| Organism: | Homo sapiens,human |
| Tissue: | Pancreas;derived from metastatic site:ascites |
| Disease: | Adenocarcinoma |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| III. Representative Data | |
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Figure 1. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly recommend purchasing from Cobioer). The number of Luminescence signal is 530±10(Mean±SD) on ASPC-1 cells while 12974128±557670(Mean±SD) on ASPC-1-Luc cells. |
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| IV. Stablity Test | |
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Figure 2. Stability test results. ASPC-1-Luc cells showed at least 32 passage stability with similar luminescence unit. |
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| V. In VIVO Data | |
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Index |
Content |
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Animal |
BALB/c Nude, 11-12weeks, Female |
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Implanted Position |
Intracranial injection |
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Injected Cell Numbers |
1×10^5/4ul |
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Matrix gel |
Without matrix gel |
1. Weight Change
Figure 3. ASPC-1-Luc Mean Body Weight ± SEM.
2. Total Flux
Figure 4. Total flux ASPC-1-Luc.
胰腺原位：
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Index |
Content |
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Animal |
NOD/SCID |
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Implanted Position |
胰腺原位 |
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Injected Cell Numbers |
5×10^5/50μL |
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Matrix gel |
Without matrix gel |
1. Weight Change
Figure 5. ASPC-1-Luc Orthotopic Tumor Model.
2. Total Flux
Figure 6. ASPC-1-Luc Orthotopic Tumor Model.
