hCDH17/MC-38
CBPG0084
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| I. Background | |||||||||||
| 钙粘蛋白超家族是一组钙依赖性膜表面糖蛋白分子，主要介导细胞粘附、胚胎发育， 并维持正常组织器官形态。CDH17 基因，即 cadherin-17，又称 liver-intestine cadherin，是 钙粘蛋白基因超家族的成员之一，编码 Ca 2+依赖性的跨膜蛋白。CDH17 作为功能性 Ca 2+依赖性细胞粘附分子发挥作用，但 是 CDH17 并不顺利获得细胞内钙连环蛋白与细胞支架的肌动蛋白方式进行紧密连接作用，而顺利获得直接与细胞支架连进行其细胞黏附作用。CDH17 结构的特异性决定了其发挥粘连作用的机制与经典的钙粘连蛋白有所不同，故 CDH17 被 划分为经典钙黏蛋白的变异体。CDH17 在人十二指肠（伯纳特腺除外）、回肠、结直肠以及阑尾上皮细胞中表达，在胃体和胃窦等组织中不表达，但在肠上皮化生的胃黏膜偶有表达。CDH17 在胃癌、结直肠癌、肝癌、胰腺癌和胆管癌等多种肿瘤组织中均有不同程度的表达，CDH17 在多种肿瘤侵袭转移中发挥重要作用。 | |||||||||||
| II. Introduction | |||||||||||
| Host Cell: | MC-38 | ||||||||||
| Expressed gene: | hCDH17 | ||||||||||
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | ||||||||||
| Freeze Medium: | 90% FBS+10% DMSO | ||||||||||
| Culture Medium: | DMEM+10%FBS+2μg/ml Puromycin | ||||||||||
| Mycoplasma Testing: | Negative | ||||||||||
| Storage: | Liquid nitrogen | ||||||||||
| III. Description of Host Cell Line | |||||||||||
| Organism: | Mus musculus, mouse | ||||||||||
| Tissue: | Colon | ||||||||||
| Morphology: | Epitheloid cell | ||||||||||
| Growth Properties: | Adherent | ||||||||||
| IV. Representative Data | |||||||||||
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Figure 1. Recombinant hCDH17/MC38 stably expressing CDH17. |
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| V. In VIVO Data | |||||||||||
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1. Weight Change
Figure 2. hCDH17/MC38 Mean Body Weight ± SEM.
2. Tumor Size
Figure 3.hCDH17/MC38 Mean Tumor Volume ± SEM.
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NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
